• Coumarin and furocoumarins

Quantification of coumarins in natural extract (Tonka broad beans, Melilot, Lavander essential oil, aromatic preparation of aniseed beverage, Pastis…)
Some of furocoumarins and psoralens are photosensitive. determination of the content of these molecules  is thus necessary in the preparations likely to contain some.
Quantification of bergapten in essentials oils of bergamot (fucoumarin free or not)
Screening for furocoumarins in essential oil and in perfumed solutions. Main coumarins and fucoumarins are Bergamottin, Epoxybergamottin, Bergapten, Byakangelicol, 7-Methoxycoumarin (herniarin), 5,7-Dimethoxycoumarin (citropten), Imperatorin, Isopimpinellin, Oxypeucedanin, Psoralen, Trioxalen, Xanthotoxin, 6-Methylcoumarin (Toncarine), SAME (5-geranoxy-7-methoxycoumarine), trioxalen, angelicin, 5-hydroxypsoralen (bergaptol), 8- hydroxypsoralen (xanthotoxol), 5-methoxyangelicin (isobergapten)…
Extraction and HPLC-DAD analysis of the 15 furocoumarins from IFRA list: Psoralen, Bergapten, Xanthotoxin, Isopimpinellin, Oxypeucedanin, Oxypeucedanin hydrate, Byakangelicol, Byakangelicin, Heraclenin, 8-geranyloxypsoralen, Bergamottin, Imperatorin, Isoimperatorin, phellopterin and Epoxybergamottin

GC Profile (polar and apolar columns)
Relative percentages of main components, characteristics of essential oils Conformity with ISO norms
Essential oil content
by steam distillation of aromatic plants
Main essential oils analysed
chinus molle, basil, bergamot, cananga, cinnamon (bark and leaf), citronnella, ginger, clove (bud, leaf, stem), lavender, palmarosa, patchouli, citrus (peal and leaf), pimento (berry and leaf), pepper, raventsara, tuberose, ylang ylang…
Realization of concretes and absolutes (to labotary scales) and characterisation
Iris: Roots, resinoïds, butter: Quantification of free irones, determination of irones potential
Clary sage: concretes, absolutes, herbs
Quantification of sclareol, epi-sclaréol and sclareolid
Tree moss – Oak moss: concretes, absolutes
Quantification of atranorin, chloratranorin, atranol, ethyl chhloratranol, ethyl hemmatomate evernyl, chlorevernyl, isopropyle hemmatomate.

Plum, Cherry plum, Quetsche, Cherry, Apricot, Prune, Peach
Determination of cyanhydric acid content using colouring method :

• in fruit spirit, spirit, infusion, maceration, extract, preserve
• white brandy may be analysed directly, in the case of coloured brandy, it is necessary to distillate first.

Regulation EEC n° 1576/89 :

• Cyanhydric acid content is limited in alcoholic beverages and beverages…
• cyanhydric acid content is limited in kernel fruits preserves.

Licorice
Determination of glycyrrhizic acid content
Method by HPLC in liquorice (Glycyrrhyza glabra L.): root, infusion, extract, powder, block, confectionery, pharmaceutical use, alcoolic beverage, Pastis.
The results are expressed with mono ammonium glycyrrhizic acid pure standard, used by DGCCRF laboratory of Montpellier for the control of flavoured spirit drinks. Labelling of main foodstuffs containing glycyrrhizic acid: particular labelling due to possible rise of hypertension (Directive CE n° 2004/77)
Glycyrrhetic acid content (isomers 18α and 18β), Enoxolone
Uses: pharmaceutical, veterinary, cosmetic